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Recent studies have also shown that high-fat diets lead to reduced mitochondrial volume, increased density, and elevated expression of the mitochondrial fission protein Drp1, which induces the expression of uncoupling protein 2 in hypothalamic microglia, increasing susceptibility to obesity (168)

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However, a breakthrough came with the observation that TICs, rather than the bulk of differentiated tumor cells, thrive in 3D gels [23, 24], offering an opportunity to uncover signaling pathways specific to TIC growth and identify TIC-specific therapeutic targets